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SLIMS |
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Inventory |
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Experiments |
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Plates
Plate are containers for compounds and other reagents. Plates are
plastic rectangles containing multiple wells that hold small quantities
of reagent, compounds, wells and so on.
In addition to the object id, SLIMS tracks all plates through a barcode
which must be unique across all plates. Each plate keeps a
reference to compounds and other reagents stored in the plate.
Each plate is defined by its platetype. By default, SLIMS
supports three types of plates
- 1536 well plates (32 rows, 48 columns)
- 384 well plates (16 rows, 24 columns)
- 96 well plates (8 rows, 12 columns)
Plates of any size can be added to slims. Some plates have
different properties than others, for example some plates have opaque
bottoms and some have translucent bottoms. If these are
particularly important for your protocol, you should define these in
the platetype inventory screen.
Additionally, each plate may have a parent_plate identifier. If a
plate has such an identifier, it automatically records all of the
reagents, compounds and so on from the parent plate. This means
if a the parent plate of plate B has compound X in well B5 then the
plate B will also contain compound X. This is used primarily for
reformating plates from mother to daughter for instance.
Double clicking on the wells entry in the plate view will show all of
the batches contained within the plate. You may have to navigate
to the mother plate for the compound list since only new compounds are
specified for daughter plates.
Available Commands
Make Dilution Protocol - Makes
a dilution protocol that can be
used to create and add dilution plates to the database. Dilution
protocols are also known as hitlist_protocols.
Add Dilution Series Plates -
This uses a dilution series or hitlist protocol to add plates to the
database. To create a dilution series plate you must specify both
the protocol to use and the compounds. A tutorial is available to step you
through this process.
Reformat - This opens up a
wizard that helps you reformat
plates. Plates can be transferred
from 96 well plates to 384 well plates or simply copied from one plate
to another. Before you reformat plates, you should have a list of the
target and destination barcodes. See here for more instructions.
Show Contents - Visually shows
the contents of the plates. This retrieves the batch information
and the batch contents along with the plate and well locations for
perusal.
Add - Add a plate to the
database. You will need to update the barcode before committing
the plate. There is currently
no easy way to specify contents of a plate. This will be fixed in
release candidate 3.
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